| Abstract |
The database of RNA structure has grown tremendously since the crystal structure analyses of
ribosomal subunits in 2000.2001. During the past year, the trend toward determining the structure of
large, complex biological RNAs has accelerated, with the analysis of three intact group I introns, A-
and B-type ribonuclease P RNAs, a riboswitch.substrate complex and other structures. The growing
database of RNA structures, coupled with efforts directed at the standardization of nomenclature and
classification of motifs, has resulted in the identification and characterization of numerous RNA
secondary and tertiary structure motifs. Because a large proportion of RNA structure can now be shown
to be composed of these recurring structural motifs, a view of RNA as a modular structure built from
a combination of these building blocks and tertiary linkers is beginning to emerge. At the same time,
however, more detailed analysis of water, metal, ligand and protein binding to RNA is revealing the
effect of these moieties on folding and structure formation. The balance between the views of RNA
structure either as strictly a construct of preformed building blocks linked in a limited number of
ways or as a flexible polymer assuming a global fold influenced by its environment will be the focus
of current and future RNA structural biology.
|