Subject: | RNA Secondary Structure |
Presenter: | Sanja Rogic |
Abstract |
Role of pre-mRNA secondary structure in splicing of Saccharomyces cerevisiae
High specificity of splice site identification by the splicing complex
can not be fully explained by primary sequence conservation. This
motivated us to look for the effects of RNA secondary structure in
splicing. We used minimum free energy secondary structure prediction as
well as structure predictions based on comparative sequence analysis to
look for conserved stems within introns. We also examined structural
characteristics of the branchpoint sequence. |